LIMS*Nucleus example database contains plate sets, plates, hit list, assay runs and data. The various projects are at different stages of completion to demonstrate the functionality of the application.
| Project ID | Description | Utility |
|---|---|---|
| PRJ-1 | 3 plate sets with 2 96 well plates each; includes data and hit lists | visualize data; visualize hit lists; group plate sets; |
| PRJ-2 | 1 plate set with 2 384 well plates each; includes data | create hit lists |
| PRJ-3 | 1 plate set with 1 1536 well plate | visualize data |
| PRJ-10 | 2 plate sets with 10 96 well plates each | add data;create hit lists; subselect plate sets |
### Files for import
Provide your own import files or use the supplied data below:
| Description | File |
|---|---|
| Accession IDSs for PS-1 ( 2 96 well plates, 4 controls) | accs96x2controls4.txt |
| ELISA data for 2 96 well plates with 4 controls col 12 (LYT-1) | sample96controls4lowp2.txt |
| HTRF data for 5 96 well plates with 8 controls col 12 (LYT-7) | sample96controls8low5plates.txt |
| A new 384 well layout with controls randomly placed | controls8scatteredNoEdge384.txt |
Statistics for example assay data files
| Assay Run | File | Response | mean(neg) | stdev(neg) | neg + 3SD |
|---|---|---|---|---|---|
| AR-1 | ar1data.txt | raw | 0.1155 | 0.07 | 0.3255 |
| bkgrnd_sub | 0.1146 | 0.0697 | 0.3236 | ||
| norm | 0.1807 | 0.1172 | 0.5322 | ||
| norm_pos | 0.1971 | 0.1236 | 0.568 | ||
| % enhanced | NA | 0 | NA | ||
| AR-2 | ar2data.txt | raw | 0.0945 | 0.0332 | 0.1942 |
| bkgrnd_sub | 0.0922 | 0.0357 | 0.1993 | ||
| norm | 0.1381 | 0.0601 | 0.3184 | ||
| norm_pos | 0.1641 | 0.0703 | 0.3751 | ||
| % enhanced | NA | NA | NA | ||
| AR-3 | ar3data.txt | raw | 2850 | 2616.2951 | 10698.8853 |
| bkgrnd_sub | 2849.9978 | 2616.2935 | 10698.8781 | ||
| norm | 0.1102 | 0.1076 | 0.4331 | ||
| norm_pos | 0.1184 | 0.1095 | 0.4469 | ||
| % enhanced | NA | NA | NA | ||
| AR-4 | ar4data.txt | raw | 0.109 | 0.066 | 0.3069 |
| bkgrnd_sub | 0.1076 | 0.066 | 0.3057 | ||
| norm | 0.1513 | 0.0956 | 0.438 | ||
| norm_pos | 0.1906 | 0.1168 | 0.5411 | ||
| % enhanced | NA | NA | NA | ||
| AR-5 | ar5data.txt | raw | 0.0764 | 0.0512 | 0.23 |
| bkgrnd_sub | 0.0728 | 0.0512 | 0.2265 | ||
| norm | 0.0895 | 0.0629 | 0.2783 | ||
| norm_pos | 0.1259 | 0.0885 | 0.3914 | ||
| % enhanced | NA | NA | NA |