Layouts

LIMS*Nucleus makes use of the following definitions:

Sample: Item of interest being tracked by LIMS*Nucleus, i.e. the item in wells. Examples would be compounds, antibodies, bacterial clones, DNA fragments, siRNAs.

Target: the item with which the sample interacts, usually coated on the bottomn of the microwell plate e.g. the antigen for an antibody or the enzyme (target) of a compound.

When creating layouts there are three attributes that need to be defined:

Entity	Attribute
Sample	type, replication
Target	replication

LIMS*Nucleus support 5 sample types:

Type	ID
unknown	1
positive control	2
negative control	3
blank	4
edge	5

LIMS*Nucleus has twenty pre-defined layouts installed at the time of system installation. Custom sample layouts can be defined and imported by administrators. A sample layout import file that defines four control wells at the bottom of column 7 looks like:

well	type
1	1
2	1
3	1
4	1
5	1
...
51	1
52	1
53	2
54	2
55	3
56	4
57	1
58	1
...

92	1
93	1
94	1
95	1
96	1

When viewed in the layout viewer, the above file would provide the following sample layout:

For every sample layout imported, an additional 5 layouts are created that define sample and target replication. These layouts are discussed in detail on the replication page.

Here is a sample layout import file that defines 8 controls in a 384 well plate, randomly scattered, excluding edge wells

When reformatted into 1536, the layout will look like: